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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin

Anti -Laminin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum IH
Laminin is a non-collagenous protein of basement membranes. This glycoprotein has a molecular weight of ~900kD. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400kD) and three short arms (200kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to Laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro.
Catalog #L1225-01E
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen, FFPE): 1:50-1:100 using the Indirect method. For frozen sections fixation with acetone for 5 min at -20°C is recommended. On paraffin embedded tissue sections proteolytic treatment with pepsin is advised. Dilute immediately before use with PBS or Tris.
Optimal dilutions to be determined by the researcher.
Positive controlSkin, kidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid.
PuritySerum
ImmunogenLaminin isolated from EHS-mouse sarcoma. Cellular Localization: Basement membrane.
SpecificityRecognizes Laminin. Does not react with human type I, III, IV and V collagen. Reacted with a distinct band of ~200-220kD from a 8M Urea extract from amion basement membrane. Species Crossreactivity: Most mammals
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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