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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Laminin B2

Anti -Laminin B2

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH
Catalog #L1227
ApplicationsSuitable for use in Immunohistochemistry (unfixed, frozen monkey brain sections). Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaMouse-rat hybridoma of X63.Ag8 653 mouse myeloma with Fisher rat splenocytes.
Immunohistochemistry 1. 10um unfixed, frozen tissue sections were washed with PBS twice, for 20 minutes.. 2. Add 400ul of 8% albumin in PBS and incubate for 1 hour at room temperature. 3. Wash the sections with PBS four times, 15 minutes each, at room temperature. 4. Incubate the sections with a 10ug/ml a-Mouse Laminin B2 Chain in 1.0% albumin in PBS and incubate overnight at 4°C. Also run a negative control (no primary antibody) to check for non-specific staining. 5. Wash the sections with PBS twice for 30 minutes. 6. Incubate the sections with a 1100 dilution of goat anti-rat IgG fluorescein conjugated secondary antibody in 1% BSA in PBS for 2 hours at room temperature.
7. Wash the section with PBS three times, 15 minutes each.
8. Examine the sections under a fluorescent microscope.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2,k
Clone No0.T.83 (A5)
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.05M Tris-glycine, pH 7.0, 40% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenMurine EHS laminin.
SpecificityRecognizes and is specific for the mouse B2 chain laminin. Does not crossreact with other basement membrane components or fibronectin.
Species crossreactivityHuman.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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