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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified IH

Catalog #	L1227
Applications	Suitable for use in Immunohistochemistry (unfixed, frozen monkey brain sections). Other applications have not been tested.
Recommended Dilution	Immunohistochemistry: 10ug/ml
	Optimal dilutions to be determined by the researcher.
Hybridoma	Mouse-rat hybridoma of X63.Ag8 653 mouse myeloma with Fisher rat splenocytes.
Immunohistochemistry 1. 10um unfixed, frozen tissue sections were washed with PBS twice, for 20 minutes.. 2. Add 400ul of 8% albumin in PBS and incubate for 1 hour at room temperature. 3. Wash the sections with PBS four times, 15 minutes each, at room temperature. 4. Incubate the sections with a 10ug/ml a-Mouse Laminin B2 Chain in 1.0% albumin in PBS and incubate overnight at 4°C. Also run a negative control (no primary antibody) to check for non-specific staining. 5. Wash the sections with PBS twice for 30 minutes. 6. Incubate the sections with a 1	100 dilution of goat anti-rat IgG fluorescein conjugated secondary antibody in 1% BSA in PBS for 2 hours at room temperature.
	7. Wash the section with PBS three times, 15 minutes each.
	8. Examine the sections under a fluorescent microscope.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2,k
Clone No	0.T.83 (A5)
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.05M Tris-glycine, pH 7.0, 40% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Murine EHS laminin.
Specificity	Recognizes and is specific for the mouse B2 chain laminin. Does not crossreact with other basement membrane components or fibronectin.
Species crossreactivity	Human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.