Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Western Blot: 2-4ug/ml detects LAT in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transfered to PVDF and probed with L1390 (2ug/ml). Proteins were visualized using IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system. Immunoprecipitation: 8-10ug immunoprecipitated LAT from 500ug of Jurkat RIPA lysate Optimal dilutions to be determined by the researcher.

J9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

GST fusion protein corresponding to residues 31-233 of human LAT.

Supplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide

Purified by Protein A affinity chromatography

Recognizes both forms of human LAT (36 and 38kD). |Species Crossreactivity: mouse