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You are here:Home » Antibodies » Antibodies-T Cells » Anti -LAT (Linker for activation of T Cells)

Anti -LAT (Linker for activation of T Cells)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Lat is an adapter protein expressed in lymphoid cells, which is a substrate for ZAP70 and Syk protein kinases. Phosphorylated LAT associates with GRB2, PLC-gamma1 and P85 PI 3-K. Expression of a mutant LAT protein missing tyrosine phosphorylation sites leads to inhibition of T-cell activation, suggesting that the protein is an important signal transducer in T-cells
Catalog #L1390
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 2-4ug/ml detects LAT in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transfered to PVDF and probed with L1390 (2ug/ml). Proteins were visualized using IgG (HRP)goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Immunoprecipitation: 8-10ug immunoprecipitated LAT from 500ug of Jurkat RIPA lysate
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlJ9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
PurityPurified by Protein A affinity chromatography
ImmunogenGST fusion protein corresponding to residues 31-233 of human LAT.
SpecificityRecognizes both forms of human LAT (36 and 38kD).
Species CrossreactivityMouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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