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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Legionella pneumophila is the causative agent of the Legionnaires' disease, a potential fatal pneumonia. It was isolated after an outbreak of pneumonia at an American legion convention in Philadelphia. The strain Philadelphia 1 is derived from the original 1976 isolate. Legionella pneumophila is found in biofilms and fresh and industrial water systems worldwide. It can survive and replicate intracellularly in free-living protozoa, often in aquatic biofilms.
Catalog #	L1663-07
Applications	Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry: Frozen and formalin-fixed paraffin embedded sections
	Immunofluorescence: 1:20 in PBS.
	Optimal dilutions to be determined by researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C. Stable for 12 months after receipt at 4°C. Do not freeze. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Reconstituted product is stable for 12 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG3
Clone No	5F241
Host	Mouse
Concentration	~0.05mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Legionella pneumophila
Specificity	Recognizes heat-resistant L. pneumophila serogroup 1-specific lipopolysaccharide antigen.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.