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L1663-07 Mouse Anti-Legionella pneumophila

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG3
Clone Number
5F241 (LPS 1-4)
Grade
Affinity Purified
Applications
IC IF IHC
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Legionella pneumophila is the causative agent of the Legionnaires' disease, a potential fatal pneumonia. It was isolated after an outbreak of pneumonia at an American legion convention in Philadelphia. The strain Philadelphia 1 is derived from the original 1976 isolate. Legionella pneumophila is found in biofilms and fresh and industrial water systems worldwide. It can survive and replicate intracellularly in free-living protozoa, often in aquatic biofilms.

Applications
Suitable for use in Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Frozen and formalin-fixed paraffin embedded sections): 1:20 Immunofluorescence (IC): 1:20 in PBS. Optimal dilutions to be determined by researcher.
Storage and Stability
Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile ddH2O. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Legionella pneumophila
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes heat-resistant L. pneumophila serogroup 1-specific lipopolysaccharide antigen.
References
1. Sethi, K.K. & Brandis, H., Establishment of Hybridoma Cell Lines Secreting Anti-Legionella pneumophila Serogroup 1 Monoclonal Antibodies with Immunodiagnostic Potential. Zbl. Bakt. Hyg., Abt. Orig. A 255: 294-298 (1983).
USBio References
No references available
Conjugates
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