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You are here:Home » Antibodies » Antibodies-Obesity Proteins » Anti -Leptin

Anti -Leptin


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Leptin, the product of the ob (obese) gene, is a 16kD protein consisting of 147 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence the reproductive function.
Catalog #L1670-21B
ApplicationsSuitable for use in ELISA and Western blotting.
Titer Defined by indirect ELISA is >1:100,000 for antibody concentration 1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.
Reconstitution Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of
the antibody. In this case clarify the solution by centrifugation.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSterile filtered and lyophilized from 1mg/ml 0.05M phosphate buffer, 0.1M sodium chloride, pH 7.2.
PurityImmunoaffinity chromatography on a column with immobilized mouse leptin.
ImmunogenRecombinant mouse leptin.
SpecificityThe recombinant mouse leptin is 100% homologous with the mouse serum leptin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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