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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Leptin, the product of the ob (obese) gene, is a 16kD protein consisting of 147 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence the reproductive function.
Catalog #	L1670-21B
Applications	Suitable for use in ELISA and Western blotting.
Titer	Defined by indirect ELISA is >1:100,000 for antibody concentration 1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.
Reconstitution	Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of
	the antibody. In this case clarify the solution by centrifugation.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Host	Rabbit
Source	Mouse
Concentration	1mg/ml
Form	Sterile filtered and lyophilized from 1mg/ml 0.05M phosphate buffer, 0.1M sodium chloride, pH 7.2.
Purity	Immunoaffinity chromatography on a column with immobilized mouse leptin.
Immunogen	Recombinant mouse leptin.
Specificity	The recombinant mouse leptin is 100% homologous with the mouse serum leptin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.