LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59kD (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483aa in length. The extracellular region contains ten LRRs (aa61-299), followed by a 73aa cytoplasmic tail (aa444-516). Over aa34-422. human LRRTM2 shares 98% aa identity with mouse LRRTM2.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.5ng/well. Western Blot: 1ug/ml Immunohistochemistry: 10ug/ml detected LRRTM2 in mouse E10.5 neural tube. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted F4598-39 followed by NLS557 IgG xSh in the dark. Between each step, cells were washed with PBS, BSA. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa34-422 of human Leucine Rich Repeat Transmembrane Protein 2, extracellular domain|expressed in NSO cells (O43300).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Leucine Rich Repeat Transmembrane Protein 2 (LRRTM2). Shows <5% crossreactivity with recombinant human LRRTM1, LRRTM3 and LRRTM4. Species Crossreactivity: Mouse.
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