Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
HeLa cell transfectants expressing human BLTR.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the human BLTR molecule, the leukotriene B4 receptor. BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.
References
1. Pettersson, A. et al. (2000). First generation monoclonal antibodies identifying the human leukotriene B receptor-1. Biochem. Biophys. Res. Commun. 279: 520-525.