| BLT1 has been reported in blood, bone marrow, brain, heart, liver, and skeletal muscle. In addition, BLT1 has been shown to be highly expressed in leukocytes and lymphoid tissues such as spleen, thymus, and lymph node. ESTs have been isolated from B-cell/lung/testis, breast, colon, eye, kidney, skin, spleen, testis, and tonsil libraries. |
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| Catalog # | L2046-01D |
| Applications | |
| Suitable for use in Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: Neat-1:2. Use 10ul to label 10e6 cells or 100ul whole blood. |
| Optimal dilution determined by the researcher |
| Hybridoma | Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| Recommended Negative Controls | I1904-84B2: IgG2a Negative Control, Murine (PE) |
| Storage and Stability | Lyophilized powder may be stored at 4°C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 6A429 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a lyphilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile ddH2O. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | HeLa cell transfectants expressing human BLTR. |
| Specificity | Recognizes the human BLTR molecule, the leukotriene B4 receptor. |
| BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
