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Abs to Transcription Factors, Homeobox (HOX)
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Anti -Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein Lhx1, MGC126723, MGC138141)
Anti -Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein
Lhx1, MGC126723, MGC138141)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
|
| LIM homeobox 1 gene product (Lim-1), also known as LHX-1, is a homeobox nuclear transcription factor expressed in human brain, thymus, tonsil and many myeloid leukemias. The 406 amino acid human LIM-1 contains a LIM domain with twin zinc-binding sequences and a homeobox DNA-binding domain. The C-terminal sequence used as an immunogen is virtually identical between human and mouse LIM-1. A truncated human isoform does not include this sequence. LIM-1 activity is controlled by interaction with the ubiquitous nuclear adaptor protein Ldb-1 (Lim-domain-binding protein-1) which binds the LIM domain and mediates dimerization. | | | | | | Applications: | | | Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. | | | Catalog # | L2200A | | Recommended Dilution | Western Blot: 1-2ug/ml | | Immunohistochemistry: 5-10ug/ml | | ELISA: 0.5-1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 7H83 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | E. coli-derived recombinant human LIM-1 (aa265-406) Accession # P48742 | | Specificity | Recognizes human LIM-1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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