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You are here:Home » Antibodies » Antibodies-Transcription Factors, Homeobox (HOX) » Anti -Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein Lhx1, MGC126723, MGC138141)

Anti -Lim-1 (Homeobox Protein Lim1, LIM Homeobox Protein 1, LHX1, LIM/Homeobox Protein
Lhx1, MGC126723, MGC138141)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
LIM homeobox 1 gene product (Lim-1), also known as LHX-1, is a homeobox nuclear transcription factor expressed in human brain, thymus, tonsil and many myeloid leukemias. The 406 amino acid human LIM-1 contains a LIM domain with twin zinc-binding sequences and a homeobox DNA-binding domain. The C-terminal sequence used as an immunogen is virtually identical between human and mouse LIM-1. A truncated human isoform does not include this sequence. LIM-1 activity is controlled by interaction with the ubiquitous nuclear adaptor protein Ldb-1 (Lim-domain-binding protein-1) which binds the LIM domain and mediates dimerization.
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.
Catalog #L2200A
Recommended DilutionWestern Blot: 1-2ug/ml
Immunohistochemistry: 5-10ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No7H83
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human LIM-1 (aa265-406) Accession # P48742
SpecificityRecognizes human LIM-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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