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Anti -LMO4 (Lim Region Containing Transcription Factor)
Anti -LMO4 (Lim Region Containing Transcription Factor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| LMO4 is a 18-20kD member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. The 165 amino acid human LMO4 comprises a trans-activating Pro-rich N-terminus, two LIM domains, and a LIM-binding C-terminus. The LIM domain is a conserved cystein-rich structure containing two tandemly repeated zinc fingers involved in protein-protein interaction. LIMs are found in a variety of proteins known to have critical functions in cell-fate specification and differentiation. LMO4 is both nuclear and cytoplasmic. LMO4 is overexpressed in most primary breast carcinomas. Human and mouse LMO4 protein sequences are 100% identical. | | | Catalog # | L3190-01 | | Applications | Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human and mouse LMO4. The detection limit for rhLMO4 and rmLMO4 is approximately 10 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively. | | Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human and mouse LMO4. The detection limit for rhLMO4 and rmLMO4 is approximately 1.5 ng/well. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml. | | Purity | Purified by human LMO4 affinity chromatography. | | Immunogen | E. coli-derived recombinant human LMO4 | | Specificity | Selected for its ability to recognize human and mouse LMO4 in direct ELISAs and Western Blots. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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