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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -LMO4 (Lim Region Containing Transcription Factor)

Anti -LMO4 (Lim Region Containing Transcription Factor)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
LMO4 is a 18-20kD member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. The 165 amino acid human LMO4 comprises a trans-activating Pro-rich N-terminus, two LIM domains, and a LIM-binding C-terminus. The LIM domain is a conserved cystein-rich structure containing two tandemly repeated zinc fingers involved in protein-protein interaction. LIMs are found in a variety of proteins known to have critical functions in cell-fate specification and differentiation. LMO4 is both nuclear and cytoplasmic. LMO4 is overexpressed in most primary breast carcinomas. Human and mouse LMO4 protein sequences are 100% identical.
Catalog #L3190-01
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human and mouse LMO4. The detection limit for rhLMO4 and rmLMO4 is approximately 10 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human and mouse LMO4. The detection limit for rhLMO4 and rmLMO4 is approximately 1.5 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
PurityPurified by human LMO4 affinity chromatography.
ImmunogenE. coli-derived recombinant human LMO4
SpecificitySelected for its ability to recognize human and mouse LMO4 in direct ELISAs and Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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