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L3190-01 Sheep Anti-LMO4 (Lim Region Containing Transcription Factor)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
P61968
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

LMO4 is a 18-20kD member of the LIM-only (LMO) subfamily of LIM domain-containing transcription factors. The 165 amino acid human LMO4 comprises a trans-activating Pro-rich N-terminus, two LIM domains, and a LIM-binding C-terminus. The LIM domain is a conserved cystein-rich structure containing two tandemly repeated zinc fingers involved in protein-protein interaction. LIMs are found in a variety of proteins known to have critical functions in cell-fate specification and differentiation. LMO4 is both nuclear and cytoplasmic. LMO4 is overexpressed in most primary breast carcinomas. Human and mouse LMO4 protein sequences are 100% identical.

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1-0.2ug/ml. The detection limit for rhLMO4 is 10ng/lane under non-reducing conditions and 2ng/lane for rmLMO4 under reducing conditions. ELISA: 0.5-1ug/ml. The detection limit for rhLMO4 and rmLMO4 is ~1.5ng/well. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Met1-Cys165 from human LMO4, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human LMO4 in direct ELISAs and Western Blots. Species Crossreactivity: mouse
Conjugates
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