| The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. |
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| Catalog # | M1202-76C |
| Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:50-1:200; Membrane permeabilization is required. |
| Immunohistochemistry (Frozen/Paraffin): 1:100-1:200; Paraffin sections require protein digestion pre-treatment (e.g. 0.1% trypsin) for 10 minutes or antigen retrieval using heat treatment prior to staining the paraffin sections. Frozen sections do not require pretreatment. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control | Human Spleen |
| Hybridoma | NS1 myeloma cells with spleen cells from BALB/c mice. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-14C: IgG, Fc (HRP) Pab Gt xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2617 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid. No stabilizing proteins or preservatives added. Endotoxin: < 0.01EU/ug. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human monocytes |
| Specificity | Recognizes the human L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. Species Crossreactivity: equine, porcine, canine, rabbit, baboon, bovine, guinea pig, rat, goat, feline, Cynomolgus monkey, Rhesus Monkey. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
