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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Macrophage, Low Endotoxin (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

Anti -Macrophage, Low Endotoxin (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
Catalog #M1202-76C
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:200; Membrane permeabilization is required.
Immunohistochemistry (Frozen/Paraffin): 1:100-1:200; Paraffin sections require protein digestion pre-treatment (e.g. 0.1% trypsin) for 10 minutes or antigen retrieval using heat treatment prior to staining the paraffin sections. Frozen sections do not require pretreatment.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlHuman Spleen
HybridomaNS1 myeloma cells with spleen cells from BALB/c mice.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2617
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid. No stabilizing proteins or preservatives added. Endotoxin: < 0.01EU/ug.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman monocytes
SpecificityRecognizes the human L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. Species Crossreactivity: equine, porcine, canine, rabbit, baboon, bovine, guinea pig, rat, goat, feline, Cynomolgus monkey, Rhesus Monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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