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M1202-76C Mouse Anti-Macrophage, Low Endotoxin (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P06702
Isotype
IgG1
Clone Number
3H2617 (MAC387)
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
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Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:200; Membrane permeabilization is required. Immunohistochemistry (Frozen/Paraffin): 1:100-1:200; Paraffin sections require protein digestion pre-treatment (e.g. 0.1% trypsin) for 10 minutes or antigen retrieval using heat treatment prior to staining the paraffin sections. Frozen sections do not require pretreatment. Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Human Spleen
Hybridoma
NS1 myeloma cells with spleen cells from BALB/c mice.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human monoocytes. UniProt Accession #: P06702
Form
Supplied as a liquid in PBS. No stabilizing proteins or preservatives added. Endotoxin: < 0.01EU/ug.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the human L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. Species Crossreactivity: equine, porcine, canine, rabbit, baboon, bovine, guinea pig, rat, goat, feline, Cynomolgus monkey, Rhesus Monkey, fallow deer, pygmy hippopotamus, mink, marmoset
References
1. Brandtzaeg, P. et al. (1988) MAC387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J. Clin. Path. 41: 963-970. 2. Brandtzaeg, P. et al. (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology. 21: 191-196. 3. Flavell, D.J. et al . (1987) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35: 1217-1226. 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques Vet. Immunol. Immunopathol. 71: 321-334. 5. Ramsay, A. D. et al. (1991) Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies. J. Pathol. 164: 321-328. 6. Christgau, M. et al. (1998) Characterization of Immunocompetent cells in the diseased canine periodontium. J. Histochem. Cytochem. 46: 1443-1454. 7. Perez, J. et al. (1999) Immunohistochemical study of the inflammatory infiltrate associated with equine squamous cell carcinoma J. Comp. Path. 121: 385-397. 8. Obert, L. et al. (2002) Early pathogenesis of transmucosal Feline Immunodeficiency Virus infection. J. Virol. 76: 6311-6322. 9. Malik, N. et al. (1998) Apoptosis and Cell proliferation after porcine coronary angioplasty. Circulation. 98: 1657-1665. 10. Bagavant, H. et al. (2002) Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)). Am. J. Pathol. 160: 141-149. 11. Sanchez, J. et al. (2011) Microscopical and Immunological Features of Tuberculoid Granulomata and Cavitary Pulmonary Tuberculosis in Naturally Infected Goats. J Comp Pathol. Feb 17. [Epub ahead of print] 12. Pomeroy, I.M. et al. (2005) Demyelinated neocortical lesions in marmoset autoimmune encephalomyelitis mimic those in multiple sclerosis. Brain. 128: 2713-21. 13. Vranckx, K. et al. (2012) Vaccination reduces macrophage infiltration in bronchus-associated lymphoid tissue in pigs infected with a highly virulent Mycoplasma hyopneumoniae strain BMC Veterinary Research 8:24. 14. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation J Clin Pathol 3 sept [epub ahead of print] 15. García­Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama). Vet Immunol Immunopathol. 149: 66-75. 16. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells. Cell Medicine. 4: 1-11. 17. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae. PLoS Negl Trop Dis. 2013 Mar; 7(3): e2138. 18. Tellez, A. et al. (2014) Experimental evaluation of efficacy and healing response of everolimus-eluting stents in the familial hypercholesterolemic swine model: a comparative study of bioabsorbable versus durable polymer stent platforms Coron Artery Dis. Mar 17. [Epub ahead of print]
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