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M1203-06B Mouse Anti-Macrophage, Monocyte Chemotactic Protein-1 (CCL2, Chemokine (C-C motif) ligand 2, C-C motif Chemokine 2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2, SMC-CF)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P13500
Isotype
IgG1
Clone Number
6D543 (5D3-F7)
Grade
Purified
Applications
E IC IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

MCP-1, also known as CCL2, is expressed by a range of cells including monocytes, vascular endothelial cells, smooth muscle cells, osteoblasts and glomeruler mesangial cells. MCP-1 plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (Frozen/paraffin): 1:250-1:500 Immunocytochemistry: 1:1000 ELISA: Capture Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human MCP-1.
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Purity
Purified
Specificity
Recognizes human MCP-1.
References
1. Peri, G., et al., J. Immunol. Methods 174: 249 (1994). 2. Locati, M., et al., J. Immunol. 168: 3557 (2002). 3. Piemonti, L., et al., Diabetes 51: 55 (2002).
USBio References
No references available
Conjugates
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