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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

Expression of macrophages is increased with maturation, with higher expression on peritoneal and alveolar macrophages. Some expression has also been observed on peripheral blood lymphocytes. The antigen recognised has a broad tissue distribution. Clone 10F28 stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer’s patches.
Catalog #	M1250-01F
Applications	Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution	Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul. Membrane permeabilisation is required.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	10F28
Host	Mouse
Source	Porcine
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified by Protein G affinity chromatography.
Immunogen	Porcine alveolar macrophages
Specificity	Recognizes porcine cells of the monocyte/macrophage lineage.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.