| Mucosal addressin cell adhesion molecule-1 (MadCAM-1) is a cell-surface immunogloblin (Ig) superfamily member composed of two extracellular Ig domains, followed by a mucin-like domain, a transmembrane domain, and a short cutoplasmic domain. The molecule is similar to ICAM1 and VCAM1. It interacts via its N-terminal Ig domain with the lymphocyte homing receptor alpha4 beta73, which plays a critical role in forming the gut-associated lymphoid system. MAdCAM-1 promotes the adhesion of T and B cells, monocytes/macrophages, and potentially eosinophils, basophils, and differentiated mast cells to the vascular endothelium. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene. The molecular weight is about 43kD, and it is highly expressed on high endothelial venules(VEC) and lamina propia venules found in the small intestine. |
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| Catalog # | M1275-03 |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line. |
| Applications | Suitable for use in Flow Cytometry, ELISA. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 10ul of the suggested working dilution to label 10e6 cells in 100ul. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Negative Controls | Mouse IgG1 Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Applications: |
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| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6A437 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. |
| Purity | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1. |
| Immunogen | Recombinant soluble MAdCAM-1-fc fusion protein |
| Specificity | Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but this clone does not recognize MAdCAM-1 in these tissues. This clone reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by the clone. This clone is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (in press). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
