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You are here:Home » Antibodies » Abs to Lectins » Anti -MBL-A (Mannose-binding Protein A, Mannan-binding Protein, Ra-reactive Factor Polysaccharide-binding Component p28B, RaRF p28B, Mbl1)

Anti -MBL-A (Mannose-binding Protein A, Mannan-binding Protein, Ra-reactive Factor
Polysaccharide-binding Component p28B, RaRF p28B, Mbl1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH IF
Mannose Binding Lectin (MBL), also called mannosebinding protein (MBP), is a calcium dependent oligomeric protein that belongs to the collectin family of proteins. It contains a collagen-like domain and a carbohydrate recognition domain enabling MBL to recognize carbohydrates (such as mannose and N-acetylglucosamine) on pathogens. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways, by using attached mannose binding lectin-associated serine proteases (MASP-2) in an antibody- and C1q-independent manner. MASP-2 permits cleavage of C4 and C2 to form a C3 convertase. Once it has bound, MBL exhibits complement-dependent antibacterial activities such as microbial opsonization and/or microbial lysis via membrane attack complexes and therefore plays an important role in innate immunity. In human, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, termed MBL-A and MBL-C. The MBL-A concentration in serum is about 6-fold lower compared to that of MBL-C... MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS- injection models. Moreover, it has been shown that MBL-A deficient mice have aberrant antigen- specific IgM responses and suffer from increased susceptibility to infection . Note that this monoclonal antibody is a calcium-dependent antibody.
Catalog #M2250-22
ApplicationsSuitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen): Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20min at 4°C to quench endogenous peroxidases. Primary antibody 8g6, 2ug/ml. As negative control rat IgG2a was used
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No13A54
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified mouse MBL-A
SpecificityRecognizes mouse MBL-A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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