| Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves chymase C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in mast cell granules within the cell and is the major secreated mast cell protease. Secreated mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation. |
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| Catalog # | M2414-01 |
| Applications | Suitable for use in Immunohistochemistry and Immunocytochemistry. Not recommended for Western Blot. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry/Immunocytochemistry: 0.5-1ug/ml; For optimal results, tissues or cytospin preparations should be fixed in Carnoy’s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. |
| Optimal dilutions to be determined by the researcher. |
| Source | Culture Supernatant |
| Positive Control | Lung, Heart, Skin, Placenta |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 2Q1506 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.1% sodium azide. Labeled with Biotin. |
| Purity | Purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography. |
| Immunogen | Chymase purified from human skin. |
| Specificity | Recognizes human mast cell chymase. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
