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You are here:Home » Antibodies » Antibodies-Cell Markers » Anti -Mast Cell Chymase (CMA1)

Anti -Mast Cell Chymase (CMA1)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Chymase-1 (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa 2-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa149-247).
Catalog #M2414-01D
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. Detection limit for rhChymase is ~1ng/well.
Western Blot: 1-2ug/ml. Detection limit for rhChymase is
~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection wil increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml from conditioned cell culture medium.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No8H183
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa20-247 from human Chymase, expressed in NSO cells (P23946). Species Sequence Homology: canine; 82%, mouse chymase; 75%
SpecificityRecognizes human Chymase at ~30kD. No cross reactivity with rmMCPT-1, rmMCPT-6, rmMCPT-7 or rmMCPT-11.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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