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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP

Chymase-1 (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa 2-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa149-247).
Catalog #	M2414-01D
Applications	Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	Direct ELISA: 0.5-1ug/ml. Detection limit for rhChymase is ~1ng/well.
	Western Blot: 1-2ug/ml. Detection limit for rhChymase is
	~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection wil increase sensitivity by 5 to 50 fold.
	Immunoprecipitation: 25ug/ml from conditioned cell culture medium.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	8H183
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant protein corresponding to aa20-247 from human Chymase, expressed in NSO cells (P23946). Species Sequence Homology: canine; 82%, mouse chymase; 75%
Specificity	Recognizes human Chymase at ~30kD. No cross reactivity with rmMCPT-1, rmMCPT-6, rmMCPT-7 or rmMCPT-11.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.