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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Mast Cell Chymase (CMA1)

Anti -Mast Cell Chymase (CMA1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Chymase-1 (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes. It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II. This promotes fibroblast proliferation and secretion of ECM. Mature human chymase is 226aa in length. It contains one peptidase S1 domain (aa 2-245). There is one potential alternate splice form. It shows a deletion of the 99 C-terminal amino acids (aa149-247).
Catalog #M2414-01D
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. Detection limit for rhChymase is ~1ng/well.
Western Blot: 1-2ug/ml. Detection limit for rhChymase is
~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection wil increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml from conditioned cell culture medium.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8H183
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa20-247 from human Chymase, expressed in NSO cells (P23946). Species sequence homology: canine; 82%, mouse chymase; 75%
SpecificityRecognizes human Chymase at ~30kD. No cross reactivity with rmMCPT-1, rmMCPT-6, rmMCPT-7 or rmMCPT-11.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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