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M2414-01G Sheep Anti-Mast Cell Protease 1 (Mcpt1, Chymase)

Specifications
References
Clone Type
Polyclonal
Host
Sheep
Source
Mouse
Swiss Prot
P11034
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mast cell protease1(Mcpt1), alsoknown as beta-chymase, is a member of the Chymase family of c chymotrypin-­like serine proteases (1). mMcpt1is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeabiliy in intestinal allergic hypersensitvity reactions (2). Its activation is completed by the removal of a two residue N-­terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human a-Chymase, Mcpt1 is capable of the conversion of angiotensinI to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminsh the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml. Validated with lysates of mouse small intestine tissue. Reducing conditions Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Glu19-Leu246 from mouse Mast Cell Protease 1, expressed in NSO cells.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Mast Cell Protease 1. <2% cross-reactivity with recombinant mouse (rm) Mcpt6, rmMcpt7, and rmMcpt11
References
1. Caughey, G.H. ( 2004) in Handbook of Proteolytic Enzymes (ed.Barrett, et al.), p.1531Academic Press, San Diego. 2. Wastling, J.M. et al.(1998)Am. J. Pat hol. 153: 491. 3. Murakami, M. et al. (1995) J. Biol Chem. 270:2218. 4. Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302: 773. 5. Tsnemi, K. et al. (2004) J. Pharmacol. Exp.Ther. 309:879. 6. Soga, Y. et al.(2004) J. Thorac. Cardiovasc. Surg.127: 72.
USBio References
No references available
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