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M2414-05X Mouse Anti-Mast Cell Chymase (BSA & Azide Free)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
3F258 (CC1)
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free

Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of chymase is estimated to be found in mast cells of skin and lung and suggests this enzyme plays a major role in mast cell mediated events. Human mast cell chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Formalin/paraffin): 1:2000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Positive Control
Tonsil
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human skin chymase. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M2414-05
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Mast Cell Chymase. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.
References
1. Buckley, M.G. et al. J. Pathol. 189:138–143.(1999)
USBio References
No references available
Conjugates
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