| Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading extracellular matrix. Stromelysin, a member of the matrix metalloproteinase family, demonstrates wide substrate specificity with the ability to degrade proteoglycan, fibronectin, laminin, casein, and the nonhelical region of collagen. The two forms of stromelysin (SL), types 1 (MMP-3) and 2 (MMP-10), share 82% sequence homology, but exhibit differences in cellular synthesis and inducibility by cytokines and growth factors in vitro. |
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| Catalog # | M2426-14 |
| MW of Antigen | ~57kD (pro form) and ~44kD (active form) |
| Epitope | Not determined |
| Applications and Recommended Dilutions | Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate) |
| Western Blotting (Ab 1-2ug/ml for 2 hrs at RT) |
| Immunohistochemistry (Formalin/paraffin) (Use Ab at 1-2ug/ml for 30 min at RT) * [No special pretreatment is required for the staining of routine formalin-fixed, paraffinembedded tissues] The optimal dilution for a specific application should be determined by the investigator. |
| Positive Control | Conditioned, serum-free medium from (TPA-treated) human large cell lymphoma U937 cells. Placenta. Bladder, breast, and ovarian carcinomas. |
| Cellular Localization | Cytoplasmic |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 3F266 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2426-14X |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human recombinant MMP-10 (stromelysin-2 protein). |
| Specificity | Recognizes pro (latent) and active forms of MMP-10. Species Crossreactivity: human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
