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M2469-02B Mouse Anti-MBD1 (Methyl CpG Binding Protein 1, MeCP1, CXXC3, MeCP1 Complex, Methyl CpG Binding Domain Protein 1, Methyl CpG Binding Protein MBD1, Protein Containing Methyl CpG Binding Domain 1, PCM1, Regulator of Fibroblast Growth Factor 2 (FGF 2) Transcription, RFT) [KO Validated]

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q9UIS9
Isotype
IgG1
Clone Number
100B272.1
Grade
Affinity Purified
Applications
WB KO
Crossreactivity
Hu
Accession #
NP_056671.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human MBD1. In HeLa, a 70kD band is observed. Mouse reactivity reported in scientific literature (PMID: 25798578)
Conjugates
Pricing
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