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Anti -MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45)
Anti -MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45,
OF45)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B |
|
| MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56-58kD member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2kD ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417aa in length. It contains an RGD motif and ASARM domain (aa416-433). In addition to its 57kD form, 70kD, 42-45kD and 30-35kD forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa147-433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively. | | | Catalog # | M2904-75 | | Applications | Suitable for use in Direct ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 1ug/ml. | | Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.5ng/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa17-433 at the etracellular domain of mouse MEPE expressed in NSO cells (AK70342). Species Sequence Homology: rat; 77%, human; 57% | | Specificity | Recognizes the extracellular domain of mouse MEPE. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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