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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45)

Anti -MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45,
OF45)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56-58kD member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2kD ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417aa in length. It contains an RGD motif and ASARM domain (aa416-433). In addition to its 57kD form, 70kD, 42-45kD and 30-35kD forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa147-433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.
Catalog #M2904-75
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to aa17-433 at the etracellular domain of mouse MEPE expressed in NSO cells (AK70342). Species Sequence Homology: rat; 77%, human; 57%
SpecificityRecognizes the extracellular domain of mouse MEPE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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