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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Methionine, Conjugated

Anti -Methionine, Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
Catalog #M3014
Applications Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Specificity Using a conjugate Methionine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds
Compound Cross-reactivity ratio (a) Methionine-G-BSA 1 Methionine-G-BSA(b) 1/> 1000 Methionine-AG-BSA 1/> 10,000 Methionine-CDI-BSA 1/> 10,000 Homocysteine-G-BSA 1/> 1000 Homocysteic acid-G-BSA 1/> 10,000 Cystathionine-G-BSA 1/> 10,000 Cysteine-G-BSA 1/> 10,000 Glutamate-G-BSA 1/> 10,000 Uncoupled methionine 1/> 10,000
(a) Methionine-G-BSA concentration/unconjugated or conjugated analogs concentration at half displacement ;
(b) : Non-reduced conjugate ;
G = Glutaraldehyde, BSA = Bovine Serum Albumin.
Recommended dilution The antiserum was tested using the free floating PAP technique on rat spinal cord. The anti-conjugated Methionine antibodies stains well between a 1/2,000-1/5,000 dilution.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
ImmunogenMethionine-Glutaraldehyde-BSA
SpecificityRecognizes protein-conjugated Methionine. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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