Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH FC

MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
Catalog #	M3885-73T
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Complement-mediate cytoxicity. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells
	Immunohistochemistry: Acetone-fixed, frozen tissue sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	34-1-2S
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Purity	Purified
Immunogen	C3H.SW mouse splenocytes
Specificity	Recognizes an epitope in the alpha3 domain that is common to H-2Kd and H-2Dd. Binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of b2 microglobulin. It crossreacts with the alpha3 domain of H-2Kb.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.