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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH FC

MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
Catalog #	M3885-73T
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Complement-mediate cytoxicity. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells
	Immunohistochemistry: Acetone-fixed, frozen tissue sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	34-1-2S
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Purity	Purified
Immunogen	C3H.SW mouse splenocytes
Specificity	Recognizes an epitope in the alpha3 domain that is common to H-2Kd and H-2Dd. Binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of b2 microglobulin. It crossreacts with the alpha3 domain of H-2Kb.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.