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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2

Anti -MHC Class 2

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP IH FC
Catalog #M3887-11D
Recommended Secondary Reagents F(ab')2 rabbit anti-rat IgG HRP conjugate, F(ab')2 Rabbit anti rat IgG: RPE
Fusion Partners Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
ApplicationsFlow Cytometry: 1:50-1:100
Immunohistochemistry
Immunoprecipitation
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2708
HostRat
SourceCanine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenCon A activated canine peripheral blood cells.
SpecificityRecognizes canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop. The antibody immunoprecipitates an antigen of 32/34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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