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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol.
The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
Catalog #	M3889-25
Applications	Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000-1:100,000
	Immunohistochemistry: Paraffin sections
	Concentrations can be measured in the range of ~0.006ug/L - 0.3ug/L.. The ELISA sensitivity is about 1000 times higher compared to UV detection.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D584
Host	Mouse
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS, sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
Specificity	Recognizes all 4-Arg microcystins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.