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M3889-25 Mouse Anti-Microcystin LR

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG1
Clone Number
6D584
Grade
Affinity Purified
Applications
E IHC WB
Shipping Temp
Blue Ice
Storage Temp
-20°C

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol. The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.

Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000-1:100,000 Concentrations can be measured in the range of ~0.006ug/L - 0.3ug/L.. The ELISA sensitivity is about 1000 times higher compared to UV detection. Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
Form
Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS, sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes all 4-Arg microcystins.
References
1. A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001) Abstract. 2. 2. C. Djediat, et al.; Toxicon 55, 531 (2010), 3. F. Gurbuz, et al.; Sci. Total Environ. 407, 4038 (2009) 4. Lu, et al.; Toxicol Sci. 103, 35 (2008), 5. M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001), Abstract; 6. A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001),
USBio References
No references available
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