With a direct competitive enzyme-linked immunosorbent assay (ELISA) using this antibody, IC50 values for microcystin-LR of 0.06ug/l have been obtained. The provisional guideline value proposed by the WHO is 1ug/l for drinking water. The detection limit for microcystin-LR is 0.006ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for this product was determined to be at least 7 x 10e10 l/mol. The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1ug/l were measured and a mean recovery of 99.9 ± 16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
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