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You are here:Home » Antibodies » Abs to Mitochondrial Proteins » Anti -Mitochondria

Anti -Mitochondria

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH IF
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.
Catalog #M3985-06
ApplicationsSuitable for use in Indirect Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Can also be used for isolation of intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads. Other applications have not been tested.
Recommended DilutionIndirect Immunofluorescence: 1:10-1:50, 30-100ul per slide well
Immunohistochemistry (paraffin-embedded tissues): 1:80 Using heart ventricle epithelium pretreated with EDTA, pH 8.0. Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells. Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
Immunohistochemistry: 1:100. Works best with organic fixatives (acetone, methanol, etc.). 2% PFA for 10-15 minutes. can be used but stronger use of formalin or 4% PFA limits staining.
Optimal dilutions to be determined by the researcher.
Positive Control COS7 cells, heart ventricle
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q2230
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS pH 7.2. No preservatives.
PurityPurified by ammonium sulfate precipitation.
ImmunogenHuman mitochondria.
SpecificityRecognizes human mitochondria. Gives mitochondrial staining on all human cell types. This pattern is best described as a spaghetti- like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells. Recognizes a 65kD protein by immunoprecipitation. Does not cross-react with rat and mouse. Epitope: surface of intact mitochondria.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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