USBio Logo

M3985-06 Mouse Anti-Mitochondria

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
2Q2230 (113-1)
Grade
Purified
Applications
IF IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

This antibody recognizes a 60kD nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.

Applications
Suitable for use in Indirect Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Can also be used for isolation of intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads. Other applications have not been tested.
Recommended Dilution
Indirect Immunofluorescence: 1:10-1:50, 30-100ul per slide well Immunohistochemistry (paraffin-embedded tissues): 1:80 Using heart ventricle epithelium pretreated with EDTA, pH 8.0. Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells. Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended. Immunohistochemistry: 1:100. Works best with organic fixatives (acetone, methanol, etc.). 2% PFA for 10-15 minutes. can be used but stronger use of formalin or 4% PFA limits staining. Optimal dilutions to be determined by the researcher.
Positive Control
COS7 cells, heart ventricle
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Surface of intact human mitochondria
Form
Supplied as a liquid in PBS pH 7.2. No preservative added.
Purity
Purified by ammonium sulfate precipitation from hybridoma supernatant.
Specificity
Recognizes human mitochondria. Gives mitochondrial staining on all human cell types. This pattern is best described as a spaghetti- like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells. Recognizes a 65kD protein by immunoprecipitation. Does not cross-react with rat and mouse. Epitope: surface of intact mitochondria.
References
1. Cell Regulation (1991) 2:1097–1113.2. J. of Neuroscience. (1998) 18(19):7674–7686. 3. Reubinoff, B.E., et al., Nature Biotechnology (2001) 19:1134–1140.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved