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M9520-13D Mouse Anti-Muscarinic Acetylcholine Receptor M2 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
B8E5
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Gp Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human muscarinic acetylcholine receptor M2 (M2 receptor) is a G protein-coupled cardiovascular receptor of ~55kD. This receptor is an integral membrane protein consisting of seven membrane spanning alpha-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein- coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. M9520-13D inhibits the beta-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. M9520-13D acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:50 detects bands of ~55, 63 and 65kD under non-reducing conditions. Immunohistochemistry (Frozen): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the second extracellular loop aa168-192, VRTVEDGECYIQFFSNAAVTFGTAI(C) of human Muscarinic Acetylcholine Receptor M2.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Muscarinic Acetylcholine Receptor M2. Reacts with the pentapeptide VRTVE (aa168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. Species Crossreactivity: rat, mouse, guinea pig.
References
1.Fu, L et al; Agonist-like activity of anti-peptide antibodies directed against an autoimmune epitope on the heart muscarinic acetylcholine receptor. Receptors Channels 1994, 2: 121 2.Elies, R et al; Immunochemical and functional characterization of an agonist-like monoclonal antibody against the M2 acetylcholine receptor. Eur J Biochem 1998, 251: 659 3.Nascimento, J et al; cGMP-mediated inhibition of cardiac L-type Ca2+ current by a monoclonal antibody against the M2 ACh receptor. Am J Physiol Cell Physiol 2001, 281: 1251.
USBio References
No references available
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