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M9520-30B Mouse Anti-Muscarinic Receptor M4 (Cholinergic Muscarinic Receptor M4, Chrm4)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P08173
Isotype
IgG1
Clone Number
2Q146 (17F10.2)
Grade
Purified
Applications
IHC IP WB
Crossreactivity
Hu Mk Mo Rt
Accession #
NM_000741.2
Shipping Temp
Blue Ice
Storage Temp
-20°C

Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.

Applications
Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: rat and monkey Western Blot: 4-10ug/ml by ECL on rat, mouse and human (weak) Immunoprecipitation: rat Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
M4 receptor i3 loop (human), fused to GST
Form
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes M4 muscarinic acetylcholine receptor. It is not reactive with the other subtypes. Species Crossreactivity: rat, mouse and monkey. Reacts weakly with human by Western Blot.
References
1. Bernard, V., et al., J. Neurosci. 19: 10,237-10,249 (1999).
USBio References
No references available
Conjugates
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