MuSK (muscle-specific kinase) is a 100kD type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family. It is found in the postsynaptic membrane of skeletal muscle motor endplates. MuSK does not bind agrin directly, but is part of an agrin receptor complex that promotes the clustering of acetylcholine receptor at the neuromuscular junction (NMJ). Over residues 22-503, human MuSK shares 92% amino acid sequence identity with mouse MuSK. Human MuSK apparently has multiple isoforms. One contains deletions at residues 307-394 and 454-461, while a second is a short soluble form that contains residues 120-209 plus a unique 24 aa C-terminal tail.
Applications
Sutable for use in Direct ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.1-0.2ug/ml using recombinant human MuSK. Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa22-503 from human MuSK, expressed in NS0 cells (O15146)
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human MuSK. Shows ~60% crossreactivity with rrMuSK.