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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -MuSK (Muscle/Skeletal Receptor Tyrosine Protein Kinase)

Anti -MuSK (Muscle/Skeletal Receptor Tyrosine Protein Kinase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
MuSK (muscle-specific kinase) is a 100kD type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family. It is found in the postsynaptic membrane of skeletal muscle motor endplates. MuSK does not bind agrin directly, but is part of an agrin receptor complex that promotes the clustering of acetylcholine receptor at the neuromuscular junction (NMJ). Over residues 22-503, human MuSK shares 92% amino acid sequence identity with mouse MuSK. Human MuSK apparently has multiple isoforms. One contains deletions at residues 307-394 and 454-461, while a second is a short soluble form that contains residues 120-209 plus a unique 24 aa C-terminal tail.
Catalog #M9530-01
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MuSK. The detection limit for rhMuSK is approximately 25 ng/lane under non-reducing and reducing conditions.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MuSK. The detection limit for rhMuSK is approximately 10 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
PurityPurified by human MuSK affinity chromatography.
ImmunogenNS0-derived rhMuSK extracellular domain
SpecificitySelected for its ability to recognize human MuSK in direct ELISAs and Western Blots. In these formats, this antibody shows ~60% crossreactivity with rrMuSK.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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