Suitable for use in Immunohistochemistry, ELISA, RIA and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents
Goat Anti Rat IgG: HRP (mouse absorbed)
Fusion Partners
Spleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bovine MBP.
Form
Supplied as a liquid with 0.09% sodium azide.
Purity
Tissue culture supernatant.
Specificity
Recognizes bovine myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 (12) has been reported as being suitable for use in Western blotting. Species Crossreactivity: Reacts widely with mammalian species including human, bovine, sheep, rabbit (3), mouse, rat, guinea pig and chicken (3).
References
1. Groome, N.P. et al. (1986). Region specific immunoassays for myelin basic protein. J. Neuroimmunol. 12: 253-264. 2. Glynn, P. et al. (1987). Basic protein dissociating from myelin membranes at physiological pH is cleaved into three major fragments. J. Neurochem. 48: 752-759. 3. Hruby, S. et al . (1987). Monoclonal antibodies reactive with myelin basic protein. Molecular Immunology. 24(12): 1359 - 1364
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