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You are here:Home » Antibodies » Abs to Myelin » Anti -Myelin Basic Protein, aa82-87 (MBP)

Anti -Myelin Basic Protein, aa82-87 (MBP)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Supernatant E IH RIA
Catalog #M9758-03P
ApplicationsSuitable for use in Immunohistochemistry, ELISA and RIA. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsGoat Anti Rat IgG: HRP (mouse absorbed)
Fusion PartnersSpleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No3H2750
HostRat
SourceBovine
ConcentrationAs reported
FormSupplied as a liquid with 0.1% sodium azide.
PurityTissue culture supernatant.
ImmunogenBovine MBP.
SpecificityReacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 has been reported as being suitable for use in Western blotting.
Species CrossreactivityReacts widely with mammalian species including human, bovine, sheep, rabbit (3), mouse, rat, guinea pig and chicken (3).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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