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Anti -Myelin Basic Protein, aa82-87 (MBP)
Anti -Myelin Basic Protein, aa82-87 (MBP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Supernatant |
E IH RIA |
|
| Catalog # | M9758-03P | | Applications | Suitable for use in Immunohistochemistry, ELISA and RIA. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry: Frozen | | Optimal dilutions to be determined by the researcher. | | Recommended Secondary Reagents | Goat Anti Rat IgG: HRP (mouse absorbed) | | Fusion Partners | Spleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 3H2750 | | Host | Rat | | Source | Bovine | | Concentration | As reported | | Form | Supplied as a liquid with 0.1% sodium azide. | | Purity | Tissue culture supernatant. | | Immunogen | Bovine MBP. | | Specificity | Reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 has been reported as being suitable for use in Western blotting. | | Species Crossreactivity | Reacts widely with mammalian species including human, bovine, sheep, rabbit (3), mouse, rat, guinea pig and chicken (3). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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