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You are here:Home » Antibodies » Abs to Myelin » Anti -Myelin Basic Protein, phosphorylated (MBP)

Anti -Myelin Basic Protein, phosphorylated (MBP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
Catalog #M9758-05
ApplicationsSuitable for use in Immunoblot, Protein Kinase Assay and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionImmunoblot: 0.5-2ug/ml. Strongly detects phosphorylated MBP. Note: Some preparations of MBP contain basal levels of phosphorylated MBP, which are detected by the M9758-05.
Protein Kinase Assay: Phosphorylated MBP was detected in a direct kinase assay using non-radioactive MAP Kinase Assay Kit, BioAssay™ (M2353) and usign MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay™ (M2353-50).
Immunocytochemistry: Reported by an indepeend lab (3).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No0.T.90
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to the human myelin basic protein sequence phosphorylated at Thr98 (tuberculin coupled).
SpecificityRecognizes human phosphorylated MBP and most common vertebrate species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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