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You are here:Home » Antibodies » Abs to Enzymes, Myeloperoxidase » Anti -Myeloperoxidase (MPO, pANCA)

Anti -Myeloperoxidase (MPO, pANCA)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH FC
MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulfide bond.
Catalog #M9760-01A
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:100. Membrane permeabilization is required.
Immunohistochemistry (Frozen): 1:1000-1:5000
Immunohistochemistry (Paraffin): 1:500-1:1000
Western Blot: Non-reducing conditions.
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagent (FC)I1904-66A: IgG, F(abí)2 (FITC) Pab Rb x Mo
Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control
Recommended Positive Control Tissue (IHC)Bone marrow
Recommended Secondary Antibodies (IHC)I1904-65W: IgG, F(abí)2 (HRP) Pab Rb x Mo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab Rb x Mo
HybridomaSpleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2762
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman Myeloperoxidase.
SpecificityRecognizes human myeloperoxidase (MPO). Recognizes native MPO in Western Blots and the heavy chain following boiling of the sample. Recognizes recombinant MPO in Western Blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukemias by Flow Cytometry following cell permeabilization.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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