Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Flow Cytometry: 1:50-1:100. Membrane permeabilization is required. Immunohistochemistry (Frozen): 1:1000-1:5000 Immunohistochemistry (Paraffin): 1:500-1:1000 Western Blot: Non-reducing conditions. Optimal working dilutions to be determined by researcher.

I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo

I1904-78R: IgG1 Murine Negative Control

Bone marrow

I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo

Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human Myeloperoxidase.

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purified by Protein G affinity chromatography.

Recognizes human myeloperoxidase (MPO). Recognizes native MPO in Western Blots and the heavy chain following boiling of the sample. Recognizes recombinant MPO in Western Blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukemias by Flow Cytometry following cell permeabilization.