| MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulfide bond. |
|
| Catalog # | M9760-01A |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:50-1:100. Membrane permeabilization is required. |
| Immunohistochemistry (Frozen): 1:1000-1:5000 |
| Immunohistochemistry (Paraffin): 1:500-1:1000 |
| Western Blot: Non-reducing conditions. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagent (FC) | I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo |
| |
| Recommended Negative Control: |
| I1904-78R: IgG1 Murine Negative Control |
| Recommended Positive Control Tissue (IHC) | Bone marrow |
| Recommended Secondary Antibodies (IHC) | I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo |
| Hybridoma | Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2762 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human Myeloperoxidase. |
| Specificity | Recognizes human myeloperoxidase (MPO). Recognizes native MPO in Western Blots and the heavy chain following boiling of the sample. Recognizes recombinant MPO in Western Blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukemias by Flow Cytometry following cell permeabilization. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
