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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

Catalog #	M9760-01E
Applications	Suitable for use in Flow cytometry. Other applications not tested.
Recommended Dilutions	Flow Cytometry (Neat): Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Detects myeloid cells following membrane permeabilization.
	Optimal working dilutions to be determined by researcher.
Hybridoma	X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Recommended Negative Controls	I1904-79D IgG1 Murine Negative Control (PE)
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2762
Host	Mouse
Source	Human
Concentration	~100 Tests/1ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 1% BSA. Conjugated to R. Phycoerythrin (RPE). Reconstitute with 1ml distilled water.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human myeloperoxiase.
Specificity	Recognizes human myeloperoxidase (MPO), an important component of azurophilic granules in neutrophils. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeabilization.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.