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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Smooth Muscle

Anti -Myosin, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #M9850-14C
Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types (MYL1 according to the HGM symbols).
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.25-0.5ug/ml
Immunohistochemistry: 0.5-1ug/ml detects smooth Myosin in formalin or acetone fixed frozen tissues and in formalin fixed, paraffin embedded tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 1.2% sodium acetate or PBS.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No10K285
HostMouse
SourceHuman
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman uterus smooth muscle extract.
SpecificityRecognizes human Myosin, Smooth Muscle. Reacts with the heavy chain of both SM2 isofoms. Species Crossreactivity: rabbit, chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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