| Catalog # | M9850-14C |
| Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types | the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types (MYL1 according to the HGM symbols). |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.25-0.5ug/ml |
| Immunohistochemistry: 0.5-1ug/ml detects smooth Myosin in formalin or acetone fixed frozen tissues and in formalin fixed, paraffin embedded tissues. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 1.2% sodium acetate or PBS.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 10K285 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Human uterus smooth muscle extract. |
| Specificity | Recognizes human Myosin, Smooth Muscle. Reacts with the heavy chain of both SM2 isofoms. Species Crossreactivity: rabbit, chicken |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
