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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Smooth Muscle, heavy chain (BSA & Azide Free)

Anti -Myosin, Smooth Muscle, heavy chain (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IF
SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
Catalog #M9850-14X
Applications Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (formalin/paraffin): 2-4ug/ml. Staining of formalin-fixed tissues requires digesting sections with trypsin at 1mg/ml PBS, 10 min at 37°C, followed by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlUterus
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.558B
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See M9850-14.
PurityPurified by Protein G chromatography.
ImmunogenCrude human uterus extract. Cellular Localization: Cytoplasmic
SpecificityRecognizes human MHC-1 (205kD) and MHC-2 (200kD) isoform.
Species crossreactivityBovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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