Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:20 Immunohistochemistry: neat Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rabbit tibilias muscle
Form
Supplied as a liquid. No preservative added.
Purity
Supernatant
Specificity
Recognizes adult skeletal myosin. Reacts with the N-terminal part (light micromyosin) of fast A (and fast B, according to species) myosin heavy chain. Species Crossreactivity: rabbit
References
1. Robelin, J., et al. Reproduction Nutrition Development 33: 25-41 (1993). 2. Marini, J.F., et al. J. Histochem. Cytochem. 37: 1721-1729 (1989). 3. Marini, J.F., et al. Neuromuscular Dis. 1(6):397-409 (1991).