Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Smooth Muscle, heavy chain

Anti -Myosin, Smooth Muscle, heavy chain

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
Catalog #M9850-16
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:50 for 20 min at RT. Staining of formalin-fixed tissues, requires boiling tissue tsections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilution determined by the researcher.
Positive ControlUterus
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F284
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M9850-16X
PurityPurified by Protein G affinity chromatography.
ImmunogenCrude human uterus extract. MW of Antigen
SpecificityRecognizes human MHC-1 (205kD) and MHC-2 (200kD) isoform and it stains the intact myoepithelial cell layers present in benign and in situ malignant breast lesions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links