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You are here:Home » Antibodies » Abs to Myosin » Anti -Myosin, Smooth Muscle Heavy Chain (BSA & Azide Free)

Anti -Myosin, Smooth Muscle Heavy Chain (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
Catalog #M9850-16X
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Formalin/paraffin): 1:25 for 60 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlUterus
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F284
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and sodium azide. See M9850-16.
PurityPurified by Protein G affinity chromatography.
ImmunogenCrude human uterus extract. Cellular Localization
SpecificityRecognizes human MHC-1 (205kD) and MHC-2 (200kD) isoform, staining the intact myoepithelial cell layers present in benign and in situ malignant breast lesions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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