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Anti -Myosin, Smooth Muscle Heavy Chain (BSA & Azide Free)
Anti -Myosin, Smooth Muscle Heavy Chain (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH |
|
| SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. | | | Catalog # | M9850-16X | | Applications | Suitable for use in Immunohistochemistry. Other applications have not been tested. | | Recommended Dilutions | | | Immunohistochemistry (Formalin/paraffin): 1:25 for 60 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Uterus | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 3F284 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and sodium azide. See M9850-16. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Crude human uterus extract. Cellular Localization | | Specificity | Recognizes human MHC-1 (205kD) and MHC-2 (200kD) isoform, staining the intact myoepithelial cell layers present in benign and in situ malignant breast lesions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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