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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -Nedd8 (Rub1)

Anti -Nedd8 (Rub1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
NEDD8 is a novel 81 amino acid polypeptide which is 60% identical and 80% homologous to ubiquitin(1,2). Ubiquitin is one of the most conserved eukaryotic proteins that can be conjugated to other proteins through a well defined enzymatic pathway. The importance of ubiquitination is underscored by its involvement in antigen processing, cell cycle regulation, degradation of tumor suppressors, receptor endocytosis, and signal transduction. This ubiquitin pathway involves three major enzymes and substrates. NEDD8 has been shown to activate and transfer to other proteins in a process analogous to ubiquitination.(3) The NEDD8 message was developmentally downregulated, and protein expression was highly enriched in the nucleus and weaker in the cytosol, whereas ubiquitin expression was detected equally well in the nucleus and cytosol(3). This antibody has been shown to detect a 6-kD NEDD8 monomer as well as a series of NEDD8 multimers (~14kD) or NEDD8-conjugated proteins (~90kD).
Catalog #N0580
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionELISA: 0.11.0ug/ml
Western Blotting: 13ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified from rabbit antiserum.
ImmunogenSynthetic peptide derived from the N-terminal sequence of human NEDD8.
SpecificitySpecific for the human NEDD8 protein. Reactivity has been confirmed with EGF-stimulated A431 cell lysate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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