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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B

NEDD8 is a novel 81 amino acid polypeptide which is 60% identical and 80% homologous to ubiquitin(1,2). Ubiquitin is one of the most conserved eukaryotic proteins that can be conjugated to other proteins through a well defined enzymatic pathway. The importance of ubiquitination is underscored by its involvement in antigen processing, cell cycle regulation, degradation of tumor suppressors, receptor endocytosis, and signal transduction. This ubiquitin pathway involves three major enzymes and substrates. NEDD8 has been shown to activate and transfer to other proteins in a process analogous to ubiquitination.(3) The NEDD8 message was developmentally downregulated, and protein expression was highly enriched in the nucleus and weaker in the cytosol, whereas ubiquitin expression was detected equally well in the nucleus and cytosol(3). This antibody has been shown to detect a 6-kD NEDD8 monomer as well as a series of NEDD8 multimers (~14kD) or NEDD8-conjugated proteins (~90kD).
Catalog #	N0580
Applications	Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution	ELISA: 0.1–1.0ug/ml
	Western Blotting: 1–3ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified from rabbit antiserum.
Immunogen	Synthetic peptide derived from the N-terminal sequence of human NEDD8.
Specificity	Specific for the human NEDD8 protein. Reactivity has been confirmed with EGF-stimulated A431 cell lysate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.