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N2150-02B Rabbit Anti-NF1, ID (Neurofibromin, Neurofibromatosis-related Protein NF-1)

Specifications
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P21359
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mutations linked to neurofibromatosis type 1 led to the identification of NF1. NF1 encodes the protein neurofibromin, which appears to be a negative regulator of the ras signal transduction pathway. In addition to type 1 neurofibromatosis, mutations in NF1 can also lead to juvenile myelomonocytic leukemia. Alternatively spliced NF1 mRNA transcripts have been isolated, although their functions, if any, remain unclear.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:20,000 Western Blot: 1:500-1:1000 Immunofluorescence: 1:100-1:500 Immunohistochemistry (Formalin fixed paraffin embedded): 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human NF1.
Form
Supplied as a liquid in PBS (without Mg2+, Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NF1. Species sequence homology: mouse and rat.
Conjugates
Pricing
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