USBio Logo

N2160-01G Mouse Anti-Neurofilament L, 68kD (NF-L)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3G203
Grade
Purified
Applications
E IC IF IHC IP WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Neurofilaments are intermediate (10-12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD). And NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, how phosphorylation likely mediates neurofilament function remains unclear. Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer’s disease, diffuse cortical Lewy body disease, Parkinson’s disease and amyotrophic lateral sclerosis (Lou Gehrig’ disease).

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 0.1-0.5ug/ml Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 1::20 Immunoprecipitation: 2-5ug Immunofluorescence (IC): 1:20 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Enzymatically dephosphorylated porcine neurofilament proteins
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified.
Specificity
Recognizes the 68kD polypeptides of human neurofilament and higher vertebrae. It specifically recognizes a phosphate-independent epitope on NF-L. Species Crossreactivity: mouse and rat.
References
1. Shaw G & Chau V :Proc.Natl Acad Sci USA 85,2854 (1988). 2. Edson KJ et al: J.Neurosci Res 30,105 (1991). 3. Trojanowski JQ J Hist Cytochem 35,999 (1987). 4 Shaw, G: In Burgoyne, RD(ed): The Neuronal Cytoskeleton, NY , Alan R. Liss Inc., pp 185–214 (1991). 5. Schmidt et al: Lab Invest, 56 282–294 (1987). 6. Tanaka, J. & Sobue, K.; J Neuroscience 14(3): 1038–1052 (1994).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved