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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IP IH

Neurofilaments are intermediate (10-12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD). And NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, how phosphorylation likely mediates neurofilament function remains unclear. Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer’s disease, diffuse cortical Lewy body disease, Parkinson’s disease and amyotrophic lateral sclerosis (Lou Gehrig’ disease).
Catalog #	N2160-01G
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 0.1-0.5ug/ml
	Immunohistochemistry (alcohol and paraformaldehyde-fixed paraffin-embedded or frozen tissue sections): 1-5ug/ml
	Western Blot: 0.1-0.5ug/ml
	Immunoprecipitation: 2-5ug
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3G203
Host	Mouse
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, before the addition of glycerol to 40%.
Purity	Purified from ascites fluid.
Immunogen	Neurofilament L
Specificity	Reacts with the 68kD polypeptides of human neurofilament and higher vertebrae. It specifically recognizes a phosphate-independent epitope on NF-L.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.