Human Neurofilament Medium Chain (NF-M) is a 102.448 kD (916 aa) expressed in CNS and PNS neurons. This cytoskeletal protein is normally phosphorylated in vivo, and its extent of phosphorylation correlates with different states of axonal growth and stabilization. These observations have led to the hypothesis that the NF-M protein cross-links adjacent neurofilament strands, and contributes to the structural integrity of the axon.
Applications
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells Western Blot: 1:5000-1:10,000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant construct containing the C-terminus of the human sequence expressed in Escherichia coli
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity
Recognizes Neurofilament, Medium Chain. Species crossreactivity: human, mouse, rat.
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NCBI
UniProt
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