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You are here:Home » Antibodies » Antibodies-Neurofilament » Anti -Neurofilament protein, Medium Chain (NF-M)

Anti -Neurofilament protein, Medium Chain (NF-M)


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Clone Host Grade Applications
Polyclonal Chicken Purified B IH IC
Human Neurofilament Medium Chain (NF-M) is a 102.448 kD (916 aa) expressed in CNS and PNS neurons. This cytoskeletal protein is normally phosphorylated in vivo, and its extent of phosphorylation correlates with different states of axonal growth and stabilization. These observations have led to the hypothesis that the NF-M protein cross-links adjacent neurofilament strands, and contributes to the structural integrity of the axon.
Catalog #N2160-02L
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells
Western Blot: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide.
ImmunogenRecombinant protein corresponding to the C-terminal of the NF-M gene product
SpecificityRecognizes Neurofilament, Medium Chain. Species crossreactivity: human, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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