| Human Neurofilament Medium Chain (NF-M) is a 102.448 kD (916 aa) expressed in CNS and PNS neurons. This cytoskeletal protein is normally phosphorylated in vivo, and its extent of phosphorylation correlates with different states of axonal growth and stabilization. These observations have led to the hypothesis that the NF-M protein cross-links adjacent neurofilament strands, and contributes to the structural integrity of the axon. |
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| Catalog # | N2160-02L |
| Applications | Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | |
| Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues |
| Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells |
| Western Blot: 1:5000-1:10,000 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken (HRP) Rb x Ch |
| I1906-12 IgY, Chicken (FITC) Rb x Ch |
| I1906-14 IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Mouse |
| Concentration | ~2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide. |
| Purity | Purified |
| Immunogen | Recombinant protein corresponding to the C-terminal of the NF-M gene product |
| Specificity | Recognizes Neurofilament, Medium Chain. Species crossreactivity: human, mouse, rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
