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You are here:Home » Antibodies » Abs to Neurofilament » Anti -Neurofilament L, 68-70kD (NF-L)

Anti -Neurofilament L, 68-70kD (NF-L)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
Catalog #N2165-08
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen and paraffin): 1:250
Do not use glutaraldehyde.
Western Blot: 1:250
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsSheep anti Rabbit IgG: HRP
Recommended Positive Control TissueBrain or Spinal Cord
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid with 0.1% sodium azide, before the addition of glycerol to 40%.
PurityNeat Serum.
ImmunogenPrimate neurofilaments.
SpecificityRecognizes neurofilament L having a low level of phosphorylation at 68/70kD. Stains neurons in both the central and peripheral nervous system. Shows < 1% crossreactivity with NF-M and 2% with NF-H. Species Crossreactivity: Rat, bovine, human, mouse and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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