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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). Two molecular forms of NmU, NmU-25 and its cleavage product (NmU-8) were initially isolated in 1985 from porcine spinal cord and then found in variety of mammals, birds, and reptile. NmU-like immunoreactivity has been detected in mammalian brain, GI-tracts of various species, and in the thyroid and endocrine cells of pituitary glands. Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recent identification of two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), as cognate receptors of NmU will help understand the physiological roles of NmU at the cellular and molecular levels. NMU receptors display a typical 7 TM domains with extracellular N-terminus and intracellular C-terminus.
NMU is present in nerves throughout the GI-tracts, corticotrophs within the anterior and lobe of rat and human pituitary glands, parafollicular cells of in rat thyroid gland, and in various regions of brain (spinal cord, hypothalamus, substantia nigra, hippocampus, amygdala). Low levels of NmU are also found in human adipose tissue, lymphocytes, and spleen.
Catalog #	N2171-80M
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-10ug/ml using ECL.
	ELISA: 1:100,000 using 50-100ng N2171-80L/well
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic 14aa peptide corresponding to human NmU within the N-terminal domain (KLH).
Specificity	Recognizes human Neuromedin U (NmU). Species Sequence Homology: No significant sequence homology with rat, mouse or NmU from other species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.