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N2171-80M Rabbit Anti-Neuromedin U (NmU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). Two molecular forms of NmU, NmU-25 and its cleavage product (NmU-8) were initially isolated in 1985 from porcine spinal cord and then found in variety of mammals, birds, and reptile. NmU-like immunoreactivity has been detected in mammalian brain, GI-tracts of various species, and in the thyroid and endocrine cells of pituitary glands. Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recent identification of two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), as cognate receptors of NmU will help understand the physiological roles of NmU at the cellular and molecular levels. NMU receptors display a typical 7 TM domains with extracellular N-terminus and intracellular C-terminus. NMU is present in nerves throughout the GI-tracts, corticotrophs within the anterior and lobe of rat and human pituitary glands, parafollicular cells of in rat thyroid gland, and in various regions of brain (spinal cord, hypothalamus, substantia nigra, hippocampus, amygdala). Low levels of NmU are also found in human adipose tissue, lymphocytes, and spleen.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml using ECL. ELISA: 1:100,000 using 50-100ng N2171-80L/well Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic 14aa peptide corresponding to human NmU within the N-terminal domain (KLH).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Neuromedin U (NmU). Species Sequence Homology: No significant sequence homology with rat, mouse or NmU from other species.
References
1. Lo G et al (1992) Mol. Endocrinol. 6, 1538-1544; 2. Minamino N et al (1988) BBRC 156, 355-360; 3. Conlon JM et al (1988) J. Neurochem. 51, 988-991; 4. Austin C et al (1994) J. Mol. Endocrinol. 14, 157; 5. Raddatz R et al (2000) J. Biol. Chem. 275, 32452; 6. Howard AD et al (2000) Nature 406, 70; 7. Fuji R et al (2000) JBC 275, 21068
USBio References
No references available
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