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You are here:Home » Antibodies » Antibodies-Nitric Oxide » Anti -NOS I (nNOS, bNOS, Nitric Oxide Synthase, NOS 1)

Anti -NOS I (nNOS, bNOS, Nitric Oxide Synthase, NOS 1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Nitric oxide (NO), a diffusible free radical gas, affects a variety of physiological functions (1, 2). NO acts as a neurotransmitter in the brain and peripheral nervous systems. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilation by releasing NO from the endothelium. NO has been implicated in neurotoxicity associated with stroke and neuro-degenerative diseases, neuronal regulation of smooth muscle including peristalsis, and penile erections. In macrophages, NO mediates tumoricidal and bactericidal actions. It mediates the actions of the excitatory neurotransmitter glutamate in stimulation of cGMP levels. Unlike typical neurotransmitters, NO is not stored in synaptic vesicles and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized from L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS I-III, M.W. ~130160kD). One group of enzymes is constitutive, agonist-triggered, dependent on Ca2+/calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/calmodulin-independent, and also inhibited by NMMA and L-NNA. It has been found in macrophages, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analysis of cDNA clones has identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I) (3), endothelial (eNOS/NOS-III) (4), and macrophage (mNOS/iNOS/NOS-II) (5). Both nNOS and eNOS are constitutive and the mNOS is inducible.
Suitable for use in Western Blot. Other applications have not been tested.
Catalog #N5350-03B
Recommended DilutionWestern Blot: 2ug/ml
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 1409-1424 (C1409NRLRSESIAFIEESK1424) of rat neuronal nitric oxide synthase (nNOS; NOS I).
SpecificityRecognizes rat nNOS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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