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Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK) (BSA & Azide Free)
Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK)
(BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH |
|
| Nucleophosmin (NPM, B23, Numatrin) is a phosphoprotein associated with cell nucleoli. Treatment of cells with cytotoxic drugs may make nucleophosmin enter nucleoplasm. It is reported that nucleophosmin shuttles continuously between cytoplasm and nucleoli to carry newly synthesized ribosomal proteins into nucleoli. NPMALK is a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in anaplastic large cell lymphoma. In acute promyelocytic leukemia NPM gene is fused to RARalpha gene in (5;17) (q33;q12) transition. | | | Catalog # | N7100-15X | | Applications | Suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. | | The optimal dilution for a specific application should be determined by the investigator. | | Positive Control | Tonsil | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG,k | | Clone No | 3F291 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See N7100-15. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 aa of ALK protein.1. | | MW of Antigen | 38kD. Epitope: N-terminal. | | Cellular Localization | Nuclear. | | Specificity | Recognizes human Nucleophosmin (NPM, B23, Numatrin). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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