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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK)

Anti -Nucleophosmin (NPM, B23, Numatrin, Anaplastic Lymphoma Kinase, p80, ALK, NPM-ALK)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
Nucleophosmin/B23 (NMP) is an abundant nucleolar phosphoprotein that accumulates in the nucleoplasm of cells. The putative function of NMP is ribosome assembly and transport. It is associated with pre-ribosomal particles and is localized in the granular region of the nucleolus. NMP has been found to be abundant in tumor growing cells as compared to normal resting cells. In addition, NPM has also been found to shift from the nucleoli to the nucleoplasm when cells are exposed to certain anticancer drugs. Studies indicate applications of NPM to include: cellular nucleolar marker and determination of anticancer drug-effects (3, 7, 10, 11), apoptosis and detection of drug-resistant cells (2,6) by NPM-translocation assay (3).
Catalog #N7100-16
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation (Native) and Immunohistochemistry
(frozen and paraffin; verified for human tissues only). Other applications not tested.
Recommended DilutionImmunofluorescence: 10ug/ml
ELISA: 1-2ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation (Native): 10ug/IP reaction
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa, K562, Jurkat, MCF-7 and HL60 cell lysates.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G215
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenNPM/B23 purified from rat hepatoma
SpecificityRecognizes the C-terminus of rat NPM/B23. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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