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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Affinity Purified B IH IF

Human occludin is an integral membrane protein specifically associated with tight junctions. These specialized regions of the plasma membrane prevent the diffusion of dissolved molecules between adjacent epithelial cells, seal off body cavities such as intestine or stomach lumen and prevent the diffusion of membrane proteins and glycolipids between the apical and basolateral regions of the plasma membrane. Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).
Catalog #	O5202-01
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:50
	Western Blot: 1:50
	Immunohistochemistry (Frozen): 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity	Purified
Immunogen	Recombinant corresponding to aa358-504 of human occludin.
Specificity	Recognizes human occludin. Species Crossreactivity: mouse, rat, canine
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.