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O5202-01 Guinea pig Anti-Occludin

Specifications
References
Clone Type
Polyclonal
Host
Guinea pig
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IF IHC WB
Crossreactivity
Ca Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human occludin is an integral membrane protein specifically associated with tight junctions. These specialized regions of the plasma membrane prevent the diffusion of dissolved molecules between adjacent epithelial cells, seal off body cavities such as intestine or stomach lumen and prevent the diffusion of membrane proteins and glycolipids between the apical and basolateral regions of the plasma membrane. Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).

Applications
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:50 Western Blot: 1:50 Immunohistochemistry (Frozen): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa358-504 of human occludin.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human occludin. Species Crossreactivity: mouse, rat and canine
References
1. Van Itallie, C et al; Occludin confers adhesiveness when expressed in fibroblasts. J Cell Sci 1997, 110: 1113.
USBio References
No references available
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