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O8064-04 Rabbit Anti-Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Serum
Applications
E IHC WB
Crossreactivity
Ca Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
BSA Free

Osteopontin is a 35kD extracellular matrix protein with a cell-binding domain. It was originally identified as a major component of the noncollagenous organic bone matrix however it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. This antibody should prove useful in studying the expression of this matrix glycoprotein in tissue homeostasis.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:50,000 Immunohistochemistry (paraffin): 1:50- 1:150. Western Blot: 1:250- 1:1000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide CKSKKFRRPDIQYPD corresponding to human|osteopontin (KLH) using maleimide.
Form
Supplied as liquid in PBS, pH7.2, 0.09% sodium azide.
Purity
Serum.
Specificity
Recognizes the full length human osteopontin protein at 66kD, C-terminal fragments of both thrombin and MMP-cleaved OPN at 32kD. Does not recognizes the 40kD N-terminal fragment. Species Crossreactivity: swine and canine.
References
1. Weber, G. F., and Cantor, H. (1996) Cytokine Growth Factor Rev. 7, 241–248. 2. Denhardt, D. T., and Chambers, A. F. (1994) J. Cell. Biochem. 56, 48–51.|3. Uede, T., Katagiri, Y., Iizuka, J., and Murakami, M. (1997) Microbiol. Immunol. 41, 641–648. 4. Liaw, L., et al., (1998) J. Clin. Invest. 101, 1468–1478. 5. Rittling, S. R., et al., (1998) J. Bone Miner. Res. 13, 1101–1111. 6. Ophascharoensuk, V., et al., (1999) Kidney Int. 56, 571–580. 7. Noiri, E., et al., (1999) Kidney Int. 56, 74–82. 8. Crawford, H. C., et al., (1999) Infect. Immun. 67, 4223–4230. 9. Yoshitake, H., et al., (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 8156–8160. 10. Ashkar, S., et al., (2000) Science 287, 860–864. 11. McCawley, L. J., and Matrisian, L. M. (2000) Mol. Med. Today 6, 149–156. Agnihotri, R., Crawford H.C., Haro, H., Matrisian, L.M., Havrda, M.C., and Liaw, L. (2001) J. Biol. Chem. 276, 28261-28267. 12. Denhardt, D. T., Lopez, C. A., Rollo, E. E., Hwang, S. M., An, X. R., and Walther, S. E. (1995) Ann. N. Y. Acad. Sci. 760, 127–142.
USBio References
No references available
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