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You are here:Home » Antibodies » Antibodies-Osteopontin » Anti -Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early T-Lymphocyte Activation 1

Anti -Osteopontin (OPN, Bone Sialoprotein I, BSP-1, Secreted Phosphoprotein 1, SPP1, Early
T-Lymphocyte Activation 1


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
Osteopontin is a 35kD extracellular matrix protein with a cell-binding domain. It was originally identified as a major component of the noncollagenous organic bone matrix however it has subsequently been demonstrated in a wide range of normal adult tissue and body fluids. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. This antibody should prove useful in studying the expression of this matrix glycoprotein in tissue homeostasis.
Catalog #O8064-04
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000
Immunohistochemistry (paraffin): 1:50- 1:150.
Western Blot: 1:250- 1:1000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined.
FormSupplied as liquid in PBS, pH7.2, 0.1% sodium azide before the addition of glycerol to 40%
ImmunogenA synthetic peptide CKSKKFRRPDIQYPD corresponding to human
osteopontin (KLH) using maleimide.
SpecificityRecognizes the full length human osteopontin protein at 66kD, C-terminal fragments of both thrombin and MMP-cleaved OPN at 32kD. Does not recognizes the 40kD N-terminal fragment. Species Crossreactivity: swine and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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