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O8075-07 Mouse Anti-Ovalbumin

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Isotype
IgG1
Clone Number
7C41
Grade
Ascites
Applications
E
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
-20°C

Chicken egg albumin (Ovalbumin) is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7kD. This sequence completely agrees with the reported m- RNA (messenger RNA) sequence. The carbohydrate and phosphate portions account for an additional 1428 and 160 grams per mole respectively, giving a total molecular weight of 44.3kD. Differential scanning calorimetry indicates the chicken egg albumin denatures at 84°C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp and 33 Glu which make it suitable for conjugation.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilutions
ELISA: 1:1000-1:10,000 Western Blot: 1:1000-1:5000 using ECL. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Chicken Egg Ovalbumin.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity
Ascites
Specificity
Recognizes chicken Ovalbumin.
Conjugates
Pricing
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